Human NF-L, His-SUMO Tag, E. coli
- Contributes to the maintenance of structural integrity and size of neurons.
- Linked to the development of motor neuron diseases, offering potential insights and therapeutic targets.
- As the predominant intermediate filament protein, it plays a critical role in ensuring proper neuronal function and overall neuronal well-being.
Sequence
A DNA sequence encoding NF-L protein was expressed with polyhistidine-SUMO tag at the N-terminus.
UnitProt ID:
P07196
Source
Escherichia coli
Endotoxin Test
<0.1 EU per 1 μg of the protein by the LAL method.
Purity:
>95% as determined by SDS-PAGE.
Form:
Lyophilized
Storage Buffer:
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution:
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Stability & Storage:
This product is stable after storage at:
• -20°C for 12 months in lyophilized state from date of receipt.
• -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Shipping Conditions:
Blue ice
A DNA sequence encoding NF-L protein was expressed with polyhistidine-SUMO tag at the N-terminus.
UnitProt ID:
P07196
Source
Escherichia coli
Endotoxin Test
<0.1 EU per 1 μg of the protein by the LAL method.
Purity:
>95% as determined by SDS-PAGE.
Form:
Lyophilized
Storage Buffer:
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution:
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Stability & Storage:
This product is stable after storage at:
• -20°C for 12 months in lyophilized state from date of receipt.
• -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Shipping Conditions:
Blue ice
Background Information
The full name of NF-L is Neurofilament light polypeptide.
NF-L is a 68 kDa protein found in the neuronal intermediate filament family. It helps maintain neuronal caliber and is linked to motor neuron diseases. NF-L is the most abundant among the three intermediate filament proteins. It consists of 542 amino acids and shares high sequence identity with mouse and rat NF-L. Its role in neuronal architecture and function makes it a target for studying neurodegenerative disorders.